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dsred2  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology dsred2
    Dsred2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dsred2/DsRed2+Antibody/pmc12957799-19-0-6
    Average 93 stars, based on 57 article reviews
    dsred2 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: The MRN complex maintains the biliary-derived hepatocytes in liver regeneration through ATR-Chk1 pathway.
    Article Snippet: .. After five times wash with PT (1% Triton X-100 in PBS), the larvae were incubated at 4 °C overnight with the following primary antibodies: Dendra2 (1:500, ABIN361314, Antibody-online), Dendra2 (1:500, TA180094, Origene), PCNA (1:500, ab29, Abcam), γH2AX (1:500, GTX127342, Gene Tex), 2F11 (1:1000, ab71286, Abcam), GFP (1:1000, ab6658, Abcam), mCherry (1:500, ARG55723, ArigoBio), DsRed2 (1:1000, sc101526, Santa Cruz), and Myc (1:1000, ab9106, Abcam). ..

    Article Title: The MRN complex maintains the biliary-derived hepatocytes in liver regeneration through ATR-Chk1 pathway
    Article Snippet: .. After five times wash with PT (1% Triton X-100 in PBS), the larvae were incubated at 4 °C overnight with the following primary antibodies: Dendra2 (1:500, ABIN361314, Antibody-online), Dendra2 (1:500, TA180094, Origene), PCNA (1:500, ab29, Abcam), γH2AX (1:500, GTX127342, Gene Tex), 2F11 (1:1000, ab71286, Abcam), GFP (1:1000, ab6658, Abcam), mCherry (1:500, ARG55723, ArigoBio), DsRed2 (1:1000, sc101526, Santa Cruz), and Myc (1:1000, ab9106, Abcam). ..

    Article Title: Contributions of biliary epithelial cells to hepatocyte homeostasis and regeneration in zebrafish
    Article Snippet: .. After being washed three times with the washing solution (1% Triton X-100 in PBS) and blocked in the blocking solution (4% BSA, 0.02% NaN3, 1% Triton X-100 in PBS) for an hour at 4C, larvae were incubated with antibodies against Dendra2 ((1:1000; AB821, Evrogen, Moscow, Russia), GFP (1:1000; ab6658, Abcam, Cambridge, MA), 2F11 (1:1000; ab71826, Abcam, Cambridge, MA), Anti-Alcam (1:50; ZN-5, ZIRC), Anti-collagen 1 (1:100; ab23730, Abcam, Cambridge, MA) and DsRed2 (1:1000; sc-101526, Santa Cruz, Dallas, TX) diluted in the blocking solution at 4C overnight. .. Then, larvae were washed five times with the washing solution for 40 minutes each and incubated with Alexa fluorescent-conjugated secondary antibodies (1:1000; Invitrogen, Grand Island, NY) diluted in the blocking solution at 4C overnight.

    Article Title: Mammalian Target of Rapamycin Complex 1 Signaling Is Required for the Dedifferentiation From Biliary Cell to Bipotential Progenitor Cell in Zebrafish Liver Regeneration.
    Article Snippet: The liver has a high regenerative capacity.. Upon 2/3 partial hepatectomy (PH), the hepatocytes proliferate and contribute to liver regeneration.. After severe liver injury when the proliferation of residual hepatocytes are blocked, the biliary epithelial cells (BECs) lose their morphology and express the hepatoblast and endoderm markers, dedifferentiate into bi-potential progenitor cells (BP-PCs), then proliferate and re-differentiate into mature hepatocytes.

    Biomarker Discovery:

    Article Title: Structural basis for protein glutamylation by the Legionella pseudokinase SidJ
    Article Snippet: .. GFP (B-2) (Cat # sc-9996, Lot# I2418, provider: Santa Cruz Biotechnology, Dilution: 1:2000) DSRed2 (Cat # sc-101526, Lot# L1714, provider: Santa Cruz Biotechnology, Dilution: 1:1000) GFP (B-2) Santa Cruz Biotechnology (sc-9996) Validation statement from the manufacturer: recommended for detection of GFP and GFP mutant fusion proteins by WB, IP, IF, FCM and ELISA. .. Validation found at provider's website- https://www.scbt.com/p/gfp-antibody-b-2 DSRed2 Santa Cruz Biotechnology (sc-101526) Validation statement from the manufacturer: recommended for detection of DsRed2 by WB and ELISA.

    Mutagenesis:

    Article Title: Structural basis for protein glutamylation by the Legionella pseudokinase SidJ
    Article Snippet: .. GFP (B-2) (Cat # sc-9996, Lot# I2418, provider: Santa Cruz Biotechnology, Dilution: 1:2000) DSRed2 (Cat # sc-101526, Lot# L1714, provider: Santa Cruz Biotechnology, Dilution: 1:1000) GFP (B-2) Santa Cruz Biotechnology (sc-9996) Validation statement from the manufacturer: recommended for detection of GFP and GFP mutant fusion proteins by WB, IP, IF, FCM and ELISA. .. Validation found at provider's website- https://www.scbt.com/p/gfp-antibody-b-2 DSRed2 Santa Cruz Biotechnology (sc-101526) Validation statement from the manufacturer: recommended for detection of DsRed2 by WB and ELISA.

    Western Blot:

    Article Title: Structural basis for protein glutamylation by the Legionella pseudokinase SidJ
    Article Snippet: .. GFP (B-2) (Cat # sc-9996, Lot# I2418, provider: Santa Cruz Biotechnology, Dilution: 1:2000) DSRed2 (Cat # sc-101526, Lot# L1714, provider: Santa Cruz Biotechnology, Dilution: 1:1000) GFP (B-2) Santa Cruz Biotechnology (sc-9996) Validation statement from the manufacturer: recommended for detection of GFP and GFP mutant fusion proteins by WB, IP, IF, FCM and ELISA. .. Validation found at provider's website- https://www.scbt.com/p/gfp-antibody-b-2 DSRed2 Santa Cruz Biotechnology (sc-101526) Validation statement from the manufacturer: recommended for detection of DsRed2 by WB and ELISA.

    Article Title: Antigen presentation plays positive roles in the regenerative response to cardiac injury in zebrafish
    Article Snippet: .. Reference: PMID: 34732708 anti-mCherry, https://encorbio.com/product/cpca-mcherry/ Manufacturer: The antibody recognizes mCherry on western blots, in appropriate cells and sections, and does not react with GFP. anti-DsRed, https://www.takarabio.com/documents/Certificate%20of%20Analysis/632496/632496-101717.pdf Manufacturer: This antibody recognizes DsRed-Express, DsRed-Express2, mCherry, DsRed2, E2-Crimson, tdTomato, mStrawberry, and mBanana, and both N- and C-terminal fusion proteins containing these fluorescent proteins in mammalian cell lysates. anti-Aldh1a2, https://www.scbt.com/p/aldh1a2-antibody-g-2 Reference: PMID: 34516874 anti-Aldh1a2, https://www.genetex.com/Product/Detail/Aldh1a2-antibody/GTX124302 4 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 Animals and other research organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research, and Sex and Gender in Research Laboratory animals Wild animals Reporting on sex Field-collected samples Ethics oversight Note that full information on the approval of the study protocol must also be provided in the manuscript. .. Novel plant genotypes Seed stocks Authentication Plants Manufacturer: reactivity: zebrafish Reference: PMID: 36513650 anti-pERK, https://www.cellsignal.com/products/primary-antibodies/phospho-p44-42-mapk-erk1-2-thr202-tyr204-d13-14-4e-xprabbit-mab/4370 Manufacturer: reactivity with many species, including zebrafish Reference: PMID: 35285802 anti-Fli1, https://www.abcam.com/products/primary-antibodies/fli1-antibody-epr4646-ab133485.html Reference: PMID: 29762122 anti-Mef2, https://www.bosterbio.com/polyclonal-anti-mef2-antibody-dz01398-1-boster.html Manufacturer: anti-Zebrafish Mef2 Antibody Reference: PMID: 36513650 anti-PCNA, https://www.scbt.com/p/pcna-antibody-pc10 Reference: PMID: 35264012 anti-Wif1, https://www.genetex.com/Product/Detail/WIF1-antibody/GTX16429 Reference: PMID: 30650349 N2.261, https://dshb.biology.uiowa.edu/N2-261 Reference: PMID: 25557620 Wild-type, transgenic and mutant zebrafish (3 to 12 months of age) used in this study were from the AB strain.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Structural basis for protein glutamylation by the Legionella pseudokinase SidJ
    Article Snippet: .. GFP (B-2) (Cat # sc-9996, Lot# I2418, provider: Santa Cruz Biotechnology, Dilution: 1:2000) DSRed2 (Cat # sc-101526, Lot# L1714, provider: Santa Cruz Biotechnology, Dilution: 1:1000) GFP (B-2) Santa Cruz Biotechnology (sc-9996) Validation statement from the manufacturer: recommended for detection of GFP and GFP mutant fusion proteins by WB, IP, IF, FCM and ELISA. .. Validation found at provider's website- https://www.scbt.com/p/gfp-antibody-b-2 DSRed2 Santa Cruz Biotechnology (sc-101526) Validation statement from the manufacturer: recommended for detection of DsRed2 by WB and ELISA.

    other:



    Blocking Assay:

    Article Title: Contributions of biliary epithelial cells to hepatocyte homeostasis and regeneration in zebrafish
    Article Snippet: .. After being washed three times with the washing solution (1% Triton X-100 in PBS) and blocked in the blocking solution (4% BSA, 0.02% NaN3, 1% Triton X-100 in PBS) for an hour at 4C, larvae were incubated with antibodies against Dendra2 ((1:1000; AB821, Evrogen, Moscow, Russia), GFP (1:1000; ab6658, Abcam, Cambridge, MA), 2F11 (1:1000; ab71826, Abcam, Cambridge, MA), Anti-Alcam (1:50; ZN-5, ZIRC), Anti-collagen 1 (1:100; ab23730, Abcam, Cambridge, MA) and DsRed2 (1:1000; sc-101526, Santa Cruz, Dallas, TX) diluted in the blocking solution at 4C overnight. .. Then, larvae were washed five times with the washing solution for 40 minutes each and incubated with Alexa fluorescent-conjugated secondary antibodies (1:1000; Invitrogen, Grand Island, NY) diluted in the blocking solution at 4C overnight.



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    Image Search Results


    Image acquisition immediately after astrocytes expressing GFP-OMM (green) and labeled with MitoTracker dye (magenta) are co-cultured with neurons expressing mito-mTagBFP2 (blue).

    Journal: Cell Reports Methods

    Article Title: MitoTracker transfers from astrocytes to neurons independently of mitochondria

    doi: 10.1016/j.crmeth.2026.101338

    Figure Lengend Snippet: Image acquisition immediately after astrocytes expressing GFP-OMM (green) and labeled with MitoTracker dye (magenta) are co-cultured with neurons expressing mito-mTagBFP2 (blue).

    Article Snippet: Sequences can be found in Methods S1. pDsRed2-Mito-7 was a gift from Michael Davidson (Addgene plasmid #55838; http://n2t.net/addgene:55838; RRID:Addgene_55838). pZac2.1-GfaABC1D-TurboID(full)-HA-GPI was a gift from Scott Soderling (Addgene plasmid #166055; http://n2t.net/addgene:166055; RRID:Addgene_166055).

    Techniques:

    Neurons expressing mTagBFP2 (blue) are incubated with ACM from astrocytes expressing GFP-OMM (green) and labeled with MitoTracker dye (magenta).

    Journal: Cell Reports Methods

    Article Title: MitoTracker transfers from astrocytes to neurons independently of mitochondria

    doi: 10.1016/j.crmeth.2026.101338

    Figure Lengend Snippet: Neurons expressing mTagBFP2 (blue) are incubated with ACM from astrocytes expressing GFP-OMM (green) and labeled with MitoTracker dye (magenta).

    Article Snippet: Sequences can be found in Methods S1. pDsRed2-Mito-7 was a gift from Michael Davidson (Addgene plasmid #55838; http://n2t.net/addgene:55838; RRID:Addgene_55838). pZac2.1-GfaABC1D-TurboID(full)-HA-GPI was a gift from Scott Soderling (Addgene plasmid #166055; http://n2t.net/addgene:166055; RRID:Addgene_166055).

    Techniques: